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放养密度对大杂交鲟生长性能的影响及生理应答机制 总被引：1，自引：0，他引：1
为探讨放养密度对鱼类生长及生理应答机制的影响规律和作用,作者以大规格大杂交鲟(达氏鳇(Huso dauricus)♀×施氏鲟(Acipenser schrenckii)♂)为实验材料,研究了平均初始体质量约为243 g/尾,放养密度分别为6(SD1组)、9(SD2组)、12(SD3组)、15 kg/m3(SD4组)条件下,不同放养密度处理70 d后的实验鱼生长性能变化及生理应答机制。结果显示,放养密度对大杂交鲟肥满度影响不显著,SD2组鱼类具有最大的特定生长率和生长效率,随着放养密度增加,日增质量显著降低(P0.05),特定生长率和生长效率下降。测定了血液甲状腺素(T4)、三碘甲腺原氨酸(T3)和皮质醇水平变化,发现放养密度能引起大杂交鲟3个血液生理指标发生显著改变;随着养殖时间推移,T3和皮质醇浓度显著升高,T4浓度显著下降(P0.05)。这些结果说明神经内分泌活动的变化引起大杂交鲟血液生理指标变化,进而影响实验鱼生长性能。因此,在该养殖条件下推荐的养殖密度为9 kg/m3。 相似文献
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鱼类甲状腺轴由下丘脑、垂体、甲状腺组成,下丘脑通过分泌促甲状腺激素释放激素、促肾上腺皮质激素释放激素调节垂体促甲状腺激素的合成和分泌,促甲状腺激素促进甲状腺滤泡合成并释放甲状腺激素(主要为甲状腺素—T4),T4运输至肝脏等外周组织中,经过脱碘转化形成生物活性更强的三碘甲腺原氨酸(T3),T3主要通过同核受体结合发挥生物学功能。甲状腺激素对鱼类胚胎发育、仔鱼变态及性别分化等早期生长发育过程具有重要调控作用:甲状腺激素为鱼类胚胎发育期所必须,外加适量甲状腺激素能够提高胚胎或仔鱼存活率;仔鱼变态过程中,某些外部形态变化及相关组织分化依赖甲状腺激素的调控,牙鲆等仔鱼变态期体内甲状腺激素水平及其受体表达量会显著升高;性腺发育期,甲状腺激素对调控鱼类性别分化方向可能具有一定作用。 相似文献
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以陇椒2号为试验材料,研究了α-萘乙酸钠对荒漠区日光温室辣椒产量和内源激素水平的影响。结果表明:叶面喷施35 mg·L-1的α-萘乙酸钠显著提高了辣椒生育后期的单果重,降低了辣椒不同生育阶段的落花落果率,改善了辣椒植株的营养条件,加速了辣椒根系和地上部分中后期的生长速率和干物质积累量,提高了辣椒不同生育阶段单株的产量。单果重和单株平均产量较对照分别提高15.76%和43.18%,落花落果率较对照降低19.77%。叶面喷施30～35 mg·L-1的α-萘乙酸钠显著降低了辣椒植株生长过程中叶片内源激素脱落酸（ABA）和吲哚乙酸（IAA）的含量,而且浓度越高,效果越明显;改变了辣椒叶片内源激素赤霉素（GA）和玉米素（ZR）的浓度和分布。辣椒植株的单株产量与叶片内源激素ZR含量正相关,与IAA和ABA的含量负相关,与GA相关性不显著。 相似文献
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Gerald L. Crow Marlin J. Atkinson Benny Ron Shannon Atkinson Arleene D. K. Skillman George T. F. Wong 《Aquatic Geochemistry》1998,4(3-4):469-480
Captive whitetip reef sharks, Triaenodon obesus,at Sea Life Park (SLP) Hawaii, Waimanalo, Hawaii develop goitre, whereas, T. obesus at the Waikiki Aquarium (WAQ), Honolulu,Hawaii do not develop goitre. To determine theeffects of natural concentrations of iodine on thereduction of goitre, two sharks from SLP with goitrewere placed in an enclosed coastal lagoon with naturalseawater. Using ultrasound measurements the twogoitres were initially 11.4 cm and 14.6 cm in depthand after 3 months decreased to 4.7 cm and 5.7 cm. Radioimmunoassay analysis of the thyroid hormoneT3 sera concentrations were initially 0.22 ng/mland 0.33 ng/ml and increased to 1.84 ng/ml after beingplaced in the lagoon. Sera T4 were initially0.93 ng/ml and 0.99 ng/ml and increased dramaticallyto 17 ng/ml and 56 ng/ml. Over the six month samplingperiod, two resident sharks in the lagoon with normalthyroids had sera T3 concentrations from 0.89ng/ml to 1.1 ng/ml, and sera T4 concentrationsfrom 3.1 ng/ml to 7.9 ng/ml. The hypothyroidcondition in the SLP goitred-sharks is likely linkedto the low environmental iodide (<0.005 M), andhigh nitrate (111 M) of SLP water. The WAQwell-water facility was characterized by anoxic waterwith high iodide (0.60 M), total iodine (1.90M), and typical nitrate (24.6 M)concentrations of interstitial groundwater. Thedifference of iodide concentration of SLP and WAQ tankwater (<0.005 M versus 0.60 M) wasdirectly related to the hydrogeology of the well-watersources. To avoid goitre in marine aquarium systems,we recommend maintaining iodide concentrations of atleast 0.15 M. 相似文献
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The cytokine network is involved in the immune system communication. As estrogens influence the cytokine expression in mammals, this study investigated the impact of exogenous estrogenic pollutants on selected cytokines in Dicentrarchus labrax. The gene expression of Interleukin 6, Tumour Necrosis Factor α, Transforming Growth Factor β1 and Interleukin 1β was assessed and accomplished with protein measurements in the blood for the last two. Impacts through 17β-estradiol mainly occurred at the beginning of organ regionalisation, thus falling together with a developmentally induced increase of Interleukin 1β and Tumour Necrosis Factor α gene expression in 102 dph fish. 17β-estradiol depressed this modification after 35 days of exposure and the cytokine gene expression tended to be generally down-regulated independently of the 17β-estradiol concentrations after 56 days of exposure. This impact was confirmed at the protein level, showing that 17β-estradiol affects the fine control of the cytokine network in sea bass. 相似文献
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The profiles of cortisol, testosterone, ll-ketotestosterone and 17α， 20β- dihydroxy-4-pregnene-3-one in mule rainbow trout reared under constant water temperature and natural photoperiod were determined by radioimmunoassay. Gonads of male rainbow trout reached maturity when the fish were two years old. Changes in the plasma levels of both sex steroid hormones and cortisol were closely related to the GSI. Plasma levels of testosterone, 11-ketotestostemne and 17a, 2013-dihydroxy 4-pregnene-3-one showed a clear peak in the annual breeding season, when the GSI reached their maxima. Plasma cortisol levels also showed clearly seasonal changes in both two- and three-year-old fish. The results suggest that the elevated plasma levels of cortisol may not just be due to stresses during the breeding season but have certain physiological functions in the reproduction of rainbow trout. 相似文献
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低温对百合鳞茎内源激素含量变化的影响 总被引：3，自引：0，他引：3
以东方百合Siberia和Tiber为试验材料,进行恒温和变温的低温处理,采用高效液相色谱法测定在不同冷藏解除休眠时期赤霉素(GA3)、吲哚乙酸(IAA)、脱落酸(ABA)和玉米素(ZT)4种内源激素含量。结果表明,恒温处理Tiber先解除休眠,同样处理同期种植的百合鳞茎,Tiber比Siberia一般早出苗7 d左右;变温处理比恒温处理ABA含量降低,说明变温处理对百合鳞茎解除休眠有促进作用,可以缩短解除休眠需要的时间,一般变温处理的比恒温处理的早8 d出苗,且变温处理有利于百合生长量的增加,Tiber茎粗和株高分别增加了23.1%、10.1%,同时Siberia分别增加了5.9%和16.6%。变温处理GA3含量Tiber高于恒温处理,Siberia低于恒温处理,品种之间对温度处理的响应有差异。百合鳞茎休眠的解除与IAA/ABA、ZT/ABA、GA3/ABA比值有关,变温Siberia和Tiber的3种比值都在60 d后逐渐升高,说明冷藏60 d可作为鳞茎解除休眠的临界点,为进一步研究百合鳞茎的休眠机理提供了重要线索。 相似文献
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The occurrence of imposex, imposition of male sex characteristics on female snails, has been extensively documented throughout the world. Tributyltin (TBT) and other organotins have been causally linked to imposex induction at levels as low as 2 ng/l. There are several proposed mechanisms of action. First, TBT has been shown to be neurotoxic and to accumulate in snail ganglia. Peptide hormones control sexual differentiation in gastropods, and one hypothesis is that TBT acts as a neurotoxin to abnormally release the peptide hormone Penis Morphogenic Factor (PMF). However, PMF has not been characterized to date. The neuropeptide APGWamide significantly induces imposex in the mud snail, Ilyanassa obsoleta, at 10−16 moles sub-cutaneous (SQ) injection over 2 weeks, and could be the PMF in this species. A second hypothesis is that TBT inhibits aromatase activity leading to increased testosterone levels and decreased estradiol. In vitro studies with snail digestive gland microsomes showed that TBT-dosed snails not exhibiting imposex had a 52% reduction in aromatase activity. Although the role of vertebrate sex steroids is not known in gastropods, it is possible that the combination of changes in peptide and steroid hormones may lead to imposex induction at extremely low doses of TBT. 相似文献
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采用温和的聚丙烯酰胺凝胶电泳（PAGE）法，从管藻目录藻刺松藻中分离到不同解离程度的光系统I（PSI）复合物，以及核心复合物CCI、捕光复合物LHCI和LHCI的亚组分，并就其多肽组成及相互关系做了研究。纯化的CCI复合物只含有66和56kDa多肽，荧光发射主峰在714－715nm；LHCI的77K荧光峰在683－684nm，没有高等植物730nm特征荧光，含有PSI复合物中的全部4种24.7-27.5kDa多肽。将中心复合物CPI再电泳，得到了只含24．5和27．2kDa多肽的LHCI亚组分。从而证实刺松藻LHCI也可分裂为含有光合色素的两个部分，它们与CCI结合的紧密程度不同。 相似文献